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Objective: To investigate the S protein cross-interference of the HB02 coronavirus strain, the Delta coronavirus strain and the Omicron coronavirus strain using the developed method of polyclonal enzyme-linked immunoassay (ELISA). Method(s): The monovalent vaccines of HB02 strain, Delta strain and Omicron strain were prepared in different ratios and combinations. The inactivated vaccines of HB02 strain, Delta strain and Omicon strain (Vero cell) S protein antigen ELISA method was used for detection, and the detection results were compared and the interference rate was calculated. At the same time, the interference rates between the monovalent antigen detection results of the three SARS-CoV-2 strains were analyzed. Result(s): The results of the two strains at different doses showed that the interference rate ranged from 4.21% to 29.53% when Delta strain was added to HB02 strain along with the increasing dose. When Omicron strain was increasingly added to HB02 strian, the interference rate ranged 4.40%~12.75%, and when HB02 strain was increasingly added to Delta strain, the interference rate ranged 56.17%~464.21%. The interference rate ranged from 6.29% to 25.91% when Omicron strain was added to Delta strain from a low to high dose. When HB02 strain was added to Omicron strain, the interference rate ranged from 32.05% to 337.22%. When Delta strain was added to Omicron strain, the interference rate ranged from 44.91% to 252.14%. The results of different doses of the three strains showed that the interference rate ranged from 2.79% to 22.86% when different doses of Delta strain+Omicron strain were added to HB02 strain. When different doses of HB02+Omicron strains were added to Delta strain, the interference rate was 67.16% to 267.95%. When different doses of HB02+Delta strains were added to Omicron strain, the interference rate was 100.66%~412.54%. In addition, the interference rates of monovalent Delta strain and Omicron strain detected by HB02 antigen detection kit were 70% and 90%, respectively. The interference rates of Delta strain antigen detection kit for monovalent HB02 strain and Omicron strain were 30% and 35%, respectively. The interference rates of Omicron strain antigen detection kit for monovalent HB02 strain and Delta strain were 5% and 10% respectively. Conclusion(s): Due to the high similarity of S protein in the HB02, Delta and Omicron COVID-19 vaccines, different strains were mixed at series ratios, and different components had different degrees of interference. When the components of the two strains were mixed at different doses, both Delta strain and Omicron strain showed positive interference to the HB02 strain, and the Delta strain showed a gradually increasing trend with the increasing Delta strain doses, while the interference rate did not change significantly with the variation of Omicron strain dose. HB02 strain showed strong positive interference to Delta strain, and the interference rate gradually increased with the increase of HB02 strain, while Omicron strain showed weak positive interference to Delta strain, and the interference rate did not change significantly with the increase of Omicron strain dose. Both HB02 strain and Delta strain showed strong positive interference to Omicron strain, and showed a stepwise increasing trend with the increase of the composition ratio of HB02 strain or Delta strain. When the three strains were mixed at different ratios, the Delta strain+Omicron strain showed positive interference to the HB02 strain, but the interference rate did not change significantly with the increase of the composition of Delta strain+Omicron strain. HB02 strain+Omicron strain showed strong positive interference to Delta strain. However, when the composition of HB02 strain and Delta strain was unchanged, the interference of Omicron strain to Delta strain did not change significantly no matter how Omicron strain changed. The results showed that HB02 strain had strong positive interference to Delta strain, but Omicron strain did not exert obvious interference to Delta strain. HB02+D lta strain showed strong positive interference to Omicron strain, and the ratio of HB02 and Delta strain increased stepwise. Different COVID-19 antigen detection kits were used to detect the monovalent COVID-19 samples of the three virus strains. Among them, HB02 antigen detection kit had the highest interference rate to Omicron strain samples, but Omicron strain antigen detection kit could effectively detect HB02 COVID-19 antigen. The Delta strain antigen detection kit could detect some COVID-19 antigens of HB02 strain and Omicron strain. Copyright © 2022, Chinese Journal of New Drugs Co. Ltd. All right reserved.
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SESSION TITLE: Hot Topics in Critical Care SESSION TYPE: Original Investigations PRESENTED ON: 10/18/2022 02:45 pm - 03:45 pm PURPOSE: Recent data from the national American Heart Association Get with the Guidelines Resuscitation registry suggests substantial hospital-to-hospital variation in airway management during in-hospital cardiac arrest (IHCA), with most patients undergoing endotracheal intubation. Less than 5% of IHCA patients receive a supraglottic airway (SGA). Over the past several years, SGAs have been studied extensively in out-of-hospital cardiac arrests (OHCA) with promising results and are widely used in the OHCA setting. In this study, we describe factors and airway characteristics at a center encouraging either SGA or endotracheal intubation (ETI) for IHCA advanced airway management. METHODS: We performed a retrospective observational study examining all cardiac arrests occurring at a multi-campus academic medical center between August 3, 2020 to July 11, 2021. Locations studied included general medical wards, telemetry units, and intensive care units (both medical and specialty ICUs, such as surgical or cardiac). Patients were excluded if they possessed an invasive airway at time of arrest, suffered an arrest in the ED or procedural areas (e.g., operating room, catheterization lab), or were SARS-CoV-2 positive. Of note, SGAs were not specifically discouraged during the COVID-19 pandemic at this institution. We compared patient, arrest, and airway characteristics between the SGA and endotracheal intubation (ETI) groups using t-tests or Fisher’s exact tests where appropriate. Given risk for confounding by indication, we did not compare patient outcomes between groups. RESULTS: A total of 97 patients were included in the study, of whom 82 (84.5%) received an advanced airway during cardiopulmonary resuscitation. Of these the initial airway was ETI in 46 (56.1%) arrests and SGA in 36 (43.9%) arrests. As compared to SGA, patients receiving ETI were younger (66.1 [±2.0] vs. 71.2 [±2.1], p=0.08), more likely to be obese (11.0% vs. 5.6%), and more likely to have pre-existing lung conditions (19.6% vs 11.1%)—although no difference reached the a priori defined α<0.5 level of significance. Other hypothesized differences were not as extreme including for body mass index (28.3 [±1.4] vs. 28.4 [±1.6]) and respiratory cause of arrest (34.8% vs. 47.2%). First pass success rate was 84.8% for ETI. Complications of airway management were rare with one patient in each group suffering vomiting, one instance of oropharyngeal bleeding in the SGA group, and one pneumothorax in the ETI group. CONCLUSIONS: At a center using both SGA and ETI during IHCA response, patients who were younger, more obese, and more commonly had underlying lung disease tended to receive ETI—although these associations were not statistically significant. Complications of both advanced airway modalities were rare. CLINICAL IMPLICATIONS: DISCLOSURES: No relevant relationships by jonathan daich No relevant relationships by Alex Li No relevant relationships by Ari Moskowitz No relevant relationships by Aron Soleiman
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Objective: To develop a sandwich ELISA method for detection of N protein in SARS-CoV-2 inactivated vaccine, and to further validate and primarily apply it for N protein qutification. Methods: The indirect ELISA method was used to assess the affinity of different anti-N protein antibodies by coating the recombinant N protein. Then the sandwich ELISA system was set up by using antibodies rN003 and rN002 as the capture and detection antibody, respectively. Also, the method for fully lysing virus to release all the N protein was confirmed. Validation of linear range, accuracy, precision, intermediate precision, specificity and LLD were obtained, and N protein content in 6 batches of SARS-CoV-2 inactivated vaccine was determined using this method. Results: N protein content has good linearty within the concentration range of 0.018~0.75 μg•mL-1 (R2>0.98), with the recovery of 80%~120% and CV <10%. N protein concentrations for 6 batches of samples accounted for 10%~20% of the total protein. All samples showed a good uniformity. Conclusions: The sandwich ELISA method for detection of N protein in SARS-CoV-2 inactivated vaccine was successfully developed with good linearty, accuracy, precision, intermediate precision, selectivity, and sensitivity, which can satisfy the N protein detection of SARS-CoV-2 inactivated vaccine.